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January 24, 2021[]

(no oral discussion of paper due to COVID-19 measures)

- Winderickx: Regulation and function of adenylate cyclase. Give a signaling pathway involving regulation of adenylate cyclase (Ca2+ and calmodulin regulation)

- Mizuno: EF hand motif and C2 domain, structure, how they bind Ca2+, examples of proteins containing one of these and how they transduce signals (EF hand: Calmodulin, SMIT1; C2 domain: classical PKCs, synaptotagmin)

- Vanden Broeck: 3 examples of function-based assays for GPCR-ligand binding, give (dis)advantages + how can you differentiate between agonists, inverse agonists and competitive antagonists?

January 17, 2021[]

-VDB : Insulin makes sure that glucose is taken up by mucsle cells, explain the major pathways.

-Mizuno : Give commen structures of kinases that enabales their enzymatic activity. How is PKC activated?

-JW: Bcl-2 family, give relation structure-function. Give the pathways where they are involved

January 21, 2020[]

- JW: paper about FHI1 (OVAC2 ortholog) and its role in yeast apoptosis: summarize the paper and link with the course material (basically explain intrinsic and extrinsic apoptosis + what position the FHI1 would have in these pathways)

- VDB: How would you perform a GPCR/7TM receptor binding assay? explain individual practical steps. Compare this with one example of a functional assay for a GPCR and explain the advantages of both.

- HM: Explain the structural properties of the EF hand and the C2 Ca binding domain and how these interact with Ca. give some examples of molecules that have these structures and how they transduce signals.

January 22, 2019[]

JW: paper about metacapsases

JVB: How to perform a ligand binding assay, a functional receptor assay, examples and what are the advantages of each?

HM: Explain SOCE and how Stim1 is involved

January 2018[]

JW: explain the molecular mechanisms of red / green color blindness. Explain why color blindness is more common in males than females. HM: Discuss ROS as signaling molecules

JANUARY 18, 2018[]

JW: paper about apoptosis and Bax proteins

JVB: bioamines and their synthesis, secretion, work or action and one example

HM: how does RTKs transduce a signal, give example or downstream signaling or RTKs

17 JAN 2018[]

JVB: paper Venus Tyrosine Kinase

JW: Schematic representation of intrinsic and extrinsic pathway + explaining what the most important components are and what they do. Briefly explain the difference between apoptosis, necrosis and necropsy.

HM: explain store operated CA entry. How is this regulated by PKC?

27 JAN 2017[]

JVDB: paper on GPCRs

JW: Bcl2 family: which proteins, why all of the same family, in which signal transduction pathways + other proteins in this signal transduction also explain.

HM: ROS: where and how formed + what does it do with proteins?

19 JAN 2017[]

JVDB: paper: https://www.ncbi.nlm.nih.gov/pubmed/24395463 "Divergent and convergent roles for insulin-like peptides in the worm, fly and mammalian nervous systems". Give a summary: give goal of the study, etc. Any questions were difficult and not course related, such as 'how can you investigate 40 different ILPs' ... (transgenesis, ...) And if that is not phenotypically distinguishable?

JW: AC, structure, regulation, function and role in signaling. Also give a detailed (example, eg in long term memory, or olfactory sensing)

HM: Discuss kinases (structure, operation, classes). Discuss PKA in detail in both active and inactive state.

18 JAN 2017[]

Winderickx: Article related to BH3 proteins related to apoptosis and autophagy. Present schematically the intrinsic and extraneous pathway of apoptosis and relate it.

Hideaki: Explain how in a cell the low concentration of calcium can be retained in the cytosol and how this changes if there are stimuli.

Vandenbroeck: Give 3 components derived from lipids, explain the regulation and relate to signal transduction.

30 AUG 2016 VM[]

W: article about BH3 proteins in protozoa. Briefly summarize this paper and compare with what you know from the course. Also show schematically the intrinsic and extrinsic pathway during this comparison.

HM: Discuss the IP3, Ca2 + mediated pathway. Also discuss the mechanism for supplementing the Ca2 + store.

JVB: How would you do a ligand binding assay for 7TM / GPCR. Also give functional assays and their pros and cons.

14 JAN 2016 NM[]

W: extrinsic and intrinsic pathways or apoptosis

HM: 3 Explain NO synthesis pathways + discuss the mechanisms behind blood circulation

JVB: paper about LGR4

14 JAN 2015[]

W: extrinsic and intrinsic pathways or apoptosis

HM: ROS signaling

JVB: paper on insulin and GLUT4

28 Jan 2013 nm[]

W: miRNA, RGS, forskolin, ripoptosome, IAP

Q: Discuss three components of the insulin growth factor pathway

L: article on histidine phosphorylation

28 Jan 2011 nm[]

1. Winderickx: Apoptosis is important in development and differentiation. Explain. And give the intrinsic and extrinsic pathway schematically.

2. Robben: Article about tyrosine phosphatases.


3. Vandenbroeck: Explain why adapter proteins are important in receptor tyrosine kinases. Give 3 examples and a bed-fusion.

20 Jan 2011 nm[]

  1. article by Vandenbroeck: insulin and Glut4 transporter
  2. Robben: discussing functions of ligand dependent calcium channels
  3. Winderickx: GAP, GEF, GoLoco, RGS and AKAP and their role in signal transduction

20 Jan 2011 vm[]

  1. Vandenbroeck: Article about gonadotropic receptors
  2. Robben: MAPKs, organization, regulation, upstream and downstream events
  3. Winderickx: regulation of adenylate cyclase

10 Sep 2010[]

  1. article by Winderickx: Yeast apoptosis - From genes to pathways
  2. Robben: MAPKs: organization, regulation, upstream and downstream events
  3. Vandenbroeck: Discuss 3 components of the conserved insulin / IGF signal transduction pathway (their molecular properties and related functions)

Jan 14, 2010[]

  1. article by Vandenbroeck
  2. seals: NO synthase and functioning thereof
  3. RGS, caspases (intrinsic and extrinsic), GoLoco

Jan 14, 2010[]

  1. article of seals,
  2. vandenbroek: experiments to investigate receptors (reporter gene, aequorin, scintillation, ...)
  3. Jorix: regulation of cAMP

28 Jan 2009[]

  1. MDL: Article NAADP
  2. JW: make a comparison between apoptosis and necrosis and schematically shows the intrinsic and extrinsic pathway
  3. JVdB: Discuss 3 components from the insulin signaling. (I had Insulin, Insulin receptor and IRS-1 and da was okay)

22 Jan 2009[]

  1. De Ley: NO and NO synthase (n)
  2. Winderickx: AKAP, Go Loco, RGS
  3. VDB: article gonadotropic receptor

29 Aug 2008[]

  1. Vandenbroeck: Article: Insulin signaling in growth control in D. Melanogaster (or something like that)
  2. Winderickx: Discuss the difference between necrosis and apoptosis. Briefly discuss the signaling pathways that are responsible for apoptosis.
  3. Deley: Discuss the difference between Ser / Thr kinases and Tyr kinases.

24 Jan 2008[]

  1. MDL: ROS
  2. JWX: color blindness, mole. mechanism
  3. VDB: article p450 and halloween genes and then some extras.

23 Jan 2008[]

  1. de ley: his phosphorylation
  2. vandenbroeck: discuss 3 components of insulin pathway
  3. winderickx: article apoptosis bring in connection with the course

15 Jan 2008[]

the ley: calcium: gradients, waves and osscillations[]

  1. winderinkx: discuss apoptosis versus necrosis and give intrinsic and extrinsic pathway
  2. vanden broeck: article about boss / sevenless pathway in R7 development at drosophila

15 Jan 2008[]

  1. De Ley: article about phosphohistidines
  2. Winderickx: Regulation of G-proteins, plus function of RGS and GoLoco. Give different classes of G-proteins plus their effector and second messengers.
  3. Vanden Broeck: Give 3 functional assays to examine G-proteins.
  4. Additional questions: Explain:
    • SOS
    • Prohormone Convertase
    • Phosphoinositide-3-kinase
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